Research Laboratory of Biophysics

1. Russian scientists have created a nanoelectrode that measures the effects of chemotherapy
2. The cells were relieved of “oxygen stress”

Patents:

1. Method for measuring oxygen concentration in subcutaneous tumor of experimental animals, RU 2725065 C1, 06/29/2020
2. Method for measuring the concentration of reactive oxygen species (ROS) in a subcutaneous tumor of living experimental animals, RU 2726074 C1, 08.07.2020
3. Pharmaceutical composition for the preparation of an injection solution for use in the treatment of magnetic hyperthermia and a method for its preparation, RU 2742196 C1, 03.02.2021

2023

1. Kechko, OI; Adzhubei, AA; Tolstova, AP; Indeykina, MI; Popov, IA; Zhokhov, SS; Gnuchev, NV; Mitkevich, VA; Makarov, AA; Kozin, SA. Molecular Mechanism of Zinc-Dependent Oligomerization of Alzheimer’s Amyloid-β with Taiwan (D7H) Mutation // nt. J. Mol. Sci. 2023, 24(14), 11241https://www.mdpi.com/1422-0067/24/14/11241

2. Bubley, A; Erofeev, A; Gorelkin, P; Beloglazkina, E; Majouga, A; Krasnovskaya, O. Tacrine-Based Hybrids: Past, Present, and Future // Int. J. Mol. Sci. 2023, 24(2), 1717; https://doi.org/10.3390/ijms24021717

3. Semkina, A; Nikitin, A; Ivanova, A; Chmelyuk, N; Sviridenkova, N; Lazareva, P; Abakumov, M. 3,4-Dihydroxiphenylacetic Acid-Based Universal Coating Technique for Magnetic Nanoparticles Stabilization for Biomedical Applications // 10.3390/jfb14090461

4. Tatiana N. Tikhonova, Anna A. Rubekina, Viktor A. Vorobev, Ekaterina A. Mefodeva, Eugene G. Maksimov, Yuri M. Efremov, Maxim E. Darvin, Peter S. Timashev, Peter V. Gorelkin, Alexander S. Erofeev, Nikolay N. Sysoev, and Evgeny A. Shirshin. Inhibition of Self-Assembling Peptide Fibrils Formation Using Thioflavin T as a Photosensitizer // Journal of Biomedical Photonics & Engineering, Vol 9, No 1 (2023) DOI: 10.18287/JBPE23.09.010304

5. Woodcock, E; Gorelkin, PV; Goff, PS; Edwards, CRW; Zhang, YJ; Korchev, Y; Sviderskaya, EV. Measuring Melanoma Nanomechanical Properties in Relation to Metastatic Ability and Anti-Cancer Drug Treatment Using Scanning Ion Conductance Microscopy // Cells 2023, 12(19), 2401; https://doi.org/10.3390/cells12192401

6. Nikita Savin, Alexander Erofeev, Vasilii Kolmogorov,ab Sergey Salikhov, Yuri Efremov, Peter Timashev, Natalia Grammatikova, Igor Levshin, Christopher Edwards, Yuri Korchev, Petr Gorelkin Scanning ion-conductance microscopy technique for studying the topography and mechanical properties of Candida parapsilosis yeast microorganisms Biomater // Sci., 2023

7. Tatiana N. Tikhonova,Vasilii S. Kolmogorov, Roman V. Timoshenko,Alexander N. Vaneev, Dana Cohen-Gerassi, Liubov A. Osminkina, Petr V. Gorelkin, Alexander S. Erofeev, Nikolay N. Sysoev, Lihi Adler-Abramovich, Evgeny A. Shirshin Sensing Cells-Peptide Hydrogel Interaction In Situ via Scanning Ion Conductance Microscopy // Cells 2022, 11(24), 4137

8. Daniil Spector, Alexander Erofeev, Peter Gorelkin, Dmitry Skvortsov, Alexander Trigub, Alina Markova, Vita Nikitina, Nikolay Ul’yanovskiy, Alexander Shtil’, Alevtina Semkina,g Ksenia Vlasova, Nikolay Zyk, Alexander Majouga, Elena Beloglazkina, Olga Krasnovskaya Biotinylated Pt(iv) prodrugs with elevated lipophilicity and cytotoxicity // Dalton Trans., 2023

9. Kseniya B. Varshavskaya, Vladimir A. Mitkevich, Alexander A. Makarov, Evgeny P. Barykin Synthetic, Cell-Derived, Brain-Derived, and Recombinant β-Amyloid: Modelling Alzheimer’s Disease for Research and Drug Development // Int. J. Mol. Sci. 2022, 23(23), 15036

10. Kolmogorov, V. S.; Erofeev, A. S.; Barykin, E. P.; Timoshenko, R. V.; Lopatukhina, E. V.; Kozin, S. A.; Gorbacheva, L. R.; Salikhov, S. V.; Klyachko, N. L.; Mitkevich, V. A.; Edwards, C. R. W.; Korchev, Y. E.; Makarov, A. A.; Gorelkin, P. V. Scanning Ion-Conductance Microscopy for Studying β-Amyloid Aggregate Formation on Living Cell Surfaces // Anal Chem 2023, 95 (43), 15943–15949. https://doi.org/10.1021/ACS.ANALCHEM.3C02806

11. Kolmogorov, V.; Erofeev, A.; Vaneev, A.; Gorbacheva, L.; Kolesov, D.; Klyachko, N.; Korchev, Y.; Gorelkin, P. Scanning Ion-Conductance Microscopy for Studying Mechanical Properties of Neuronal Cells during Local Delivery of Glutamate // Cells 2023, Vol. 12, Page 2428 2023, 12 (20), 2428. https://doi.org/10.3390/CELLS12202428

12. Dubkov, S.; Overchenko, A.; Novikov, D.; Kolmogorov, V.; Volkova, L.; Gorelkin, P.; Erofeev, A.; Parkhomenko, Y. Single-Cell Analysis with Silver-Coated Pipette by Combined SERS and SICM // Cells 2023, Vol. 12, Page 2521 2023, 12 (21), 2521. https://doi.org/10.3390/CELLS12212521

13. Pleskova, S. N.; Erofeev, A. S.; Vaneev, A. N.; Gorelkin, P. V.; Bobyk, S. Z.; Kolmogorov, V. S.; Bezrukov, N. A.; Lazarenko, E. V. ROS Production by a Single Neutrophil Cell and Neutrophil Population upon Bacterial Stimulation // Biomedicines 2023, 11 (5). https://doi.org/10.3390/biomedicines11051361

14. Ponomarev, V. A.; Sheveyko, A. N.; Kuptsov, K. A.; Sukhanova, E. V.; Popov, Z. I.; Permyakova, E. S.; Slukin, P. V.; Ignatov, S. G.; Ilnitskaya, A. S.; Gloushankova, N. A.; Timoshenko, R. V.; Erofeev, A. S.; Kuchmizhak, A. A.; Shtansky, D. V. X-Ray and UV Irradiation-Induced Reactive Oxygen Species Mediated Antibacterial Activity in Fe and Pt Nanoparticle-Decorated Si-Doped TiCaCON Films // ACS Appl Mater Interfaces 2023. https://doi.org/10.1021/acsami.3c13242

15. Popova, A. D.; Sheveyko, A. N.; Kuptsov, K. A.; Advakhova, D. Yu.; Karyagina, A. S.; Gromov, A. V.; Krivozubov, M. S.; Orlova, P. A.; Volkov, A. V.; Slukin, P. V.; Ignatov, S. G.; Shubina, I. Zh.; Ilnitskaya, A. S.; Gloushankova, N. A.; Timoshenko, R. V.; Erofeev, A. S.; Shtansky, D. V. Osteoconductive, Osteogenic, and Antipathogenic Plasma Electrolytic Oxidation Coatings on Titanium Implants with BMP-2 // ACS Appl Mater Interfaces 2023, 15 (31), 37274–37289. https://doi.org/10.1021/acsami.3c08954

16. Vaneev, A. N.; Timoshenko, R. V.; Gorelkin, P. V.; Klyachko, N. L.; Erofeev, A. S. Recent Advances in Nanopore Technology for Copper Detection and Their Potential Applications // Nanomaterials 2023, 13 (9), 1573. https://doi.org/10.3390/nano13091573

17. Savin, N.; Erofeev, A.; Timoshenko, R.; Vaneev, A.; Garanina, A.; Salikhov, S.; Grammatikova, N.; Levshin, I.; Korchev, Y.; Gorelkin, P. Investigation of the Antifungal and Anticancer Effects of the Novel Synthesized Thiazolidinedione by Ion-Conductance Microscopy // Cells 2023, 12 (12), 1666. https://doi.org/10.3390/cells12121666

18. Savin, N.; Erofeev, A.; Gorelkin, P. Analytical Models for Measuring the Mechanical Properties of Yeast // Cells 2023, 12 (15), 1946. https://doi.org/10.3390/cells12151946

19. Savin, N.; Erofeev, A.; Kolmogorov, V.; Salikhov, S.; Efremov, Y.; Timashev, P.; Grammatikova, N.; Levshin, I.; Edwards, C.; Korchev, Y.; Gorelkin, P. Scanning Ion-Conductance Microscopy Technique for Studying the Topography and Mechanical Properties of Candida Parapsilosis Yeast Microorganisms // Biomater Sci 2023, 11 (2), 611–617. https://doi.org/10.1039/D2BM00964A

2019-2022

1. Novotortsev Vladimir K., Kukushkin Maxim E., Tafeenko Viktor A., Skvortsov Dmitry A., Kalinina Marina A., Timoshenko Roman V., Chmelyuk Nelly S., Vasilyeva Liliya A., Tarasevich Boris N., Gorelkin Petr V., Erofeev Alexander S., Majouga Alexander G., Zyk Nikolai V., Beloglazkina Elena K. " Dispirooxindoles Based on 2-Selenoxo-Imidazolidin-4-Ones: Synthesis, Cytotoxicity and ROS Generation Ability" // Journal of Molecular Sciences (2021), том 22, № 5, с. 1-26
2. Savin N, Kolmogorov V, Iakovlev A, Timoshenko R, Gorelkin P, Erofeev A, Korchev Y “Antimicrobial Activity of Antifungal Drugs on Candida Parapsilosis Studied by Scanning Ionconductance Microscopy (SICM)” // Biophysical Journal (2021), 120:361a
3. Kolmogorov V, Vaneev A, Efremov Y, Savin N, Iakovlev A, Alova A, Lavrushkina S, Erofeev A, Gorelkin P, Klyachko N, Kireev I, Majouga A, Novak P, Korchev Y “Studying the Local Young’s Modulus of PC-3 Cells Via Scanning Ion-Conductance Microscopy” // Biophysical Journal (2021), 120:162a.
4. Timoshenko R, Vaneev A, Savin N, Chmelyuk N, Krasnovskaya O, Majouga A, Gorelkin P, Erofeev A “Nanocapillary-Based Sensors for Detection of Copper Ions in Biological Systems” // Biophysical Journal (2021), 120:89a-90a.
5. Iakovlev A, Novak P, Erofeev A, Gorelkin P, Kolmogorov V, Savin N “Comparison of Different Scanning Ion-Conductance Microscopy Methods to Study Local Mechanical Properties of Living Cells” // Biophysical Journal (2021), 20: 360a-361a.
6. Kolmogorov V, Erofeev A, Woodcock E, Efremov Y, Iakovlev A, Savin N, Alova A, Lavrushkina S, Kireev I, Prelovskaya A, Sviderskaya E, Scaini D, Klyachko N, Timashev P, Takahashi Y, Salikhov S, Parkhomenko Y, Majouga A, Edwards C, Novak P, Korchev Y, Gorelkin P “Mapping mechanical properties of living cells at nanoscale using intrinsic nanopipette-sample force interaction” // Nanoscale (2021)
7. Timoshenko R, Vaneev A, Savin N, Klyachko N, Parkhomenko Y, Salikhov S, Majouga A, Gorelkin P, Erofeev A “Promising Approaches for Determination of Copper Ions in Biological Systems” // Nanotechnologies in Russia (2020), 15:121–134.
8. Yamansarov, E.Y.; Lopatukhina, E. V.; Evteev, S.A.; Skvortsov, D.A.; Lopukhov, A. V.; Kovalev, S. V.; Vaneev, A.N.; Shkil’, D.O.; Akasov, R.A.; Lobov, A.N.; et al. “Discovery of Bivalent GalNAc-Conjugated Betulin as a Potent ASGPR-Directed Agent against Hepatocellular Carcinoma” // Bioconjug. Chem. (2021), acs.bioconjchem.1c00042
9. David Klenerman, Yuri Korchev, Pavel Novak, and Andrew Shevchuk “Noncontact Nanoscale Imaging of Cells” // Annual Review of Analytical Chemistry (2021)
10. Pamela Swiatlowska, Jose L. Sanchez-Alonso, Catherine Mansfield, Denis Scaini,bc Yuri Korchev, Pavel Novak and Julia Gorelik “Short-term angiotensin II treatment regulates cardiac nanomechanics via microtubule modifications” // Nanoscale (2020), 12, 16315-16329
11. P.Gorelkin, A.Erofeev, A. Shevchuk, P. Novak, C. Edwards, Y. Korchev “Scanning Ion Conductance Microscopy for Single Cell Analysis” // Microscopy and Microanalysis (2020)
12. Joanna Bednarska, Annegret Pelchen-Matthews, Pavel Novak, Jemima J. Burden, Peter A. Summers, Marina K. Kuimova, Yuri Korchev, Mark Marsh, and Andrew Shevchuk “Rapid formation of human immunodeficiency virus-like particles” // PNAS (2020), 117 (35) 21637-21646
13. J. Bednarska P. Novak Y. Korchev P. Rorsman A.I. Tarasov Andrew Shevchuk “Release of insulin granules by simultaneous, high‐speed correlative SICM‐FCM” // Journal of Microscopy (2020)
14. Vaneev, A.; Timoshenko, R.; Kolmogorov, V.; Lopatukhina, E.; Chmelyuk, N.; Yamansarov, E.; Petrov, R.; Majouga, A.; Korchev, Y.; Novak, P.; et al. “Electrochemical Method for Real-Time ROS Measurements in Single Cells” // Biophysical Journal (2021), 120, 365a
15. Krasnovskaya Olga, Naumov Alexey, Guk Dmitry, Gorelkin Peter, Erofeev Alexander, Beloglazkina Elena, Majouga Alexander “Copper Coordination Compounds as Biologically Active Agents” // International Journal of Molecular Sciences (2020), том 21, № 11, стр. 3965
16. Vaneev Alexander N., Gorelkin Petr V., Garanina Anastasiia S., Lopatukhina Helena V., Vodopyanov Stepan S., Alova Anna V., Ryabaya Oxana O., Akasov Roman A., Zhang Yanjun, Novak Pavel, Salikhov Sergey V., Abakumov Maxim A., Takahashi Yasufumi, Edwards Christopher R.W., Klyachko Natalia L., Majouga Alexander G., Korchev Yuri E., Erofeev Alexander S. “In Vitro and In Vivo Electrochemical Measurement of Reactive Oxygen Species After Treatment with Anticancer Drugs” // Analytical Chemistry (2020)
17. Krasnovskaya Olga, Spector Daniil, Zlobin Alexander, Pavlov Kirill, Gorelkin Peter, Erofeev Alexander, Beloglazkina Elena, Majouga Alexander “Metals in Imaging of Alzheimer’s Disease” // International Journal of Molecular Sciences (2020), том 21, № 23, стр. 9190-10025
18. Petrov Rostislav A., Mefedova Sofiia R., Yamansarov Emil Yu, Maklakova Svetlana Yu, Grishin Dmitrii A., Lopatukhina Elena V., Burenina Olga Y., Lopukhov Anton V., Kovalev Sergey V., Timchenko Yury V., Ondar Evgenia E., Ivanenkov Yan A., Evteev Sergei A., Vaneev Alexander N., Timoshenko Roman V., Klyachko Natalia L., Erofeev Alexander S., Gorelkin Petr V., Beloglazkina Elena K., Majouga Alexander G. “New small-molecule glycoconjugates of docetaxel and GalNAc for targeted delivery to hepatocellular carcinoma” // Molecular Pharmaceutics (2020)
19. Krasnovskaya Olga, Guk Dmitry A., Naumov Alexey, Nikitina Vita N., Semkina Alevtina, Vlasova Kseniya Yu, Pokrovsky Vadim, Ryabaya Oksana O., Karshieva Saida, Skvortsov Dmitry A., Zhirkina Irina, Shafikov Radik R., Gorelkin Petr, Vaneev Alexander, Erofeev Alexander, Mazur Dmitrii, Tafeenko Viktor Aleksandrovich, Pergushov Vladimir I., Melnikov Mikhail, Soldatov Mikhail A., Shapovalov Victor V., Soldatov Alexander V., Akasov Roman, Gerasimov Vasily, Sakharov Dmitry A., Moiseeva Anna A., Zyk Nikolay Vasil’evich, Beloglazkina Elena K., Majouga Alexander G. “Novel copper-containing cytotoxic agents based on 2-thioxoimidazolones” // Journal of Medicinal Chemistry (2020), том 63, № 21, стр. 13031-13063
20. Anna Alova, Alexander Erofeev, Petr Gorelkin, Tatyana Bibikova, Yury Korchev, Alexander Majouga, Alexander Bulychev “Prolonged oxygen depletion in microwounded cells of Chara corallina detected with novel O2 nanosensors” // Journal of Experimental Botany (2020), том 71, № 1, стр. 386-398
21. Reshitko Galina S., Emil Yamansarov, Sergei Evteev, Lopatukhina Elena V., Dmitriy Shkil, Saltykova Irina V., Sergey Kovalev, Anton Lopukhov, Lobov Alexander N., Ivan Kislyakov, Burenina Olga Yu, Klyachko Natalia L., Garanina Anastasiia S., Dontsova Olga A., Ivanenkov Yan A., Alexander Erofeev, Petr Gorelkin, Beloglazkina Elena K., Majouga Alexander G. “Synthesis and evaluation of new trivalent ligands for hepatocyte targeting via the asialoglycoprotein receptor” // Bioconjugate Chemistry (2020), том 31, № 5, стр. 1313-1319
22. Pershina Alexandra G., Brikunova Olga Ya, Demin Alexander M., Abakumov Maxim A., Vaneev Alexander N., Naumenko Victor A., Erofeev Alexander S., Gorelkin Peter V., Nizamov Timur R., Muslimov Albert R., Timin Alexander S., Malkeyeva Dina, Kiseleva Elena, Vtorushin Sergey V., Larionova Irina V., Gereng Elena A., Minin Artem S., Murzakaev Aidar M., Krasnov Victor P., Majouga Alexander G., Ogorodova Ludmila M “Variation in tumor pH affects pH-triggered delivery of peptide-modified magnetic nanoparticles” // Nanomedicine: Nanotechnology, Biology, and Medicine (2020), том 32
23. Tagirova, M.A.; Eremeev, N.L.; Vaneev, A.N.; Balabushevich, N.G.; Aleksashkin, A.D.; Zaitseva, E.A.; Klyachko, N.L. “Synthesis of a Two-Layer Polyionic Bienzyme Complex of Superoxide Dismutase 1 and Catalase of the Smallest Size” // Moscow University Chemistry Bulletin (2020), 75, 238–242
24. Abakumova, T.; Prikazchikova, T.; Aparin, I.; Vaneev, A.; Gorelkin, P.; Erofeev, A.; Zatsepin, T. “ROS-sensitive Dyes in Lipid Nanoparticles for in vivo Imaging” // In Proceedings of the 2020 IEEE 10th International Conference Nanomaterials: Applications & Properties (NAP); IEEE, (2020), pp. 02BA05-1-02BA05-3
25. Medvedeva M., Barinova K., Melnikova A., Semenyuk P., Kolmogorov V., Gorelkin P., Erofeev A., Muronetz V. “Naturally occurring cinnamic acid derivatives prevent amyloid transformation of alpha-synuclein” // Biochimie (2020), том 170, с. 128-139
26. Gorelkin Petr, Erofeev Alexander, Kolmogorov Vasilii, Efremov Yuri, Novak Pavel, Shevchuk Andrew, Majouga Alexander, Korchev Yuri “Scanning Ion Conductance Microscopy (SICM) for Low-stress Directly Examining of Cellular Mechanics” // Microscopy and Microanalysis (2020), том 26, № S2, с. 1968-1970
27. Yanjun Zhang, Yasufumi Takahashi, Sung Pil Hong, Fengjie Liu, Joanna Bednarska, Philip S. Goff, Pavel Novak, Andrew Shevchuk, Sahana Gopal, Iros Barozzi, Luca Magnani, Hideki Sakai, Yoshimoto Suguru, Takuto Fujii, Alexander Erofeev, Peter Gorelkin, Alexander Majouga, Dominik J. Weiss, Christopher Edwards, Aleksandar P. Ivanov, David Klenerman, Elena V. Sviderskaya, Joshua B. Edel & Yuri Korchev, “High-resolution label-free 3D mapping of extracellular pH of single living cells” // Nat. Commun. (2019), 10, 5610
28. Akasov R.A., Sholina N.V., Khochenkov D.A., Alova A.V., Gorelkin P.V., Erofeev A.S., Generalova A.N., Khaydukov E.V. “Photodynamic therapy of melanoma by blue-light photoactivation of flavin mononucleotide” // Scientific reports (2019), том 9, № 1
29. Erofeev, A.; Gorelkin, P.; Edwards, C.; Vaneev, A.; Kolmogorov, V.; Korchev, Y.; Majouga, A.; Novak, P. “Novel Biophysics Methodologies for Single Cell Analysis with Electrochemical Nanoprobes” // European Biophysics Journal (2019), 48, S243.
30. Gorelkin, P.; Erofeev, A.; Kolmogorov, V.; Vaneev, A.; Alova, A.; Shevchuk, A.; Korchev, Y.; Majouga, A.; Edwards, C.; Parkhomenko, Y.; et al. “Nanoscale Bioimaging with Scanning Ion Conductance Microscopy” // European Biophysics Journal (2019), 48, S157.
31. Vaneev, A.; Lopatuhina, E.; Alova, A.; Erofeev, A.; Gorelkin, P.; Krasnovskaya, O.; Kolmogorov, V.; Korchev, Y.; Majouga, A.; Edwards, C.; et al. “A new in vitro screening system for evaluating of anticancer drugs effect” // European Biophysics Journal (2019), 48, S241.
32. Vaneev, A.; Lopatuhina, E.; Alova, A.; Erofeev, A.; Gorelkin, P.; Krasnovskaya, O.; Kolmogorov, V.; Korchev, Y.; Majouga, A.; Edwards, C.; et al. “Novel rapid method for evaluating of anticancer drugs effect on tumor cells” // FEBS Open Bio (2019), 9, 169–170.
33. Akasov, R.; Vaneev, A.; Alova, A.; Garanina, A.; Erofeev, A.; Gorelkin, P.; Korchev, Y.; Markvicheva, E.; Majouga, A. “Electrochemical nanoprobe for label-free and real-time analyses of 3D tumor spheroids” // FEBS Open Bio (2019), 9, 387.
34. Garanina, A.; Vaneev, A.; Erofeev, A.; Gorelkin, P.; Naumenko, V.; Kolmogorov, V.; Akasov, R.; Abakumov, M.; Korchev, Y.; Majouga, A. “Novel method for intravital investigation of anticancer drug pharmacokinetics and metabolite detection in mice tumors” // FEBS Open Bio (2019), 9, 361
35. Barinova Kseniya V., Medvedeva Mariia V., Melnikova Aleksandra K., Erofeev Alexander S., Gorelkin Peter V., Kolmogorov Vasily S., Muronetz Vladimir I. “Changes in the Structure of Amyloid Alpha-Synuclein Aggregatesunder the Action of Cinnamic Acid Derivatives” // International Journal of Biomedicine (2019), том 9, № S1, с. 31
36. Lavrushkina S., Ovsiannikova N., Yudina A., Kolmogorov V., Gorelkin P., Strelkova O., Zhironkina O., Perepelina K., Malashicheva A., Kireev I. “Mutations in different domains of lamin a change the mechanical properties of the nucleus” // Biopolymers and Cell (2019), том 35, № 3, с. 220-221
37. Gorelkin P., Erofeev A., Kolmogorov V., Vaneev A., Alova A., Shevchuk A., Korchev Y., Majouga A., Edwards C., Parkhomenko Y., Salihov S., Novak P. “Nanoscale bio-imaging with scanning ion conductance microscopy” // European Biophysics Journal (2019), том 48, № Suppl. 1, с. S157-S157